Method for synthesizing phloroglucinol by virtue of in vitro enzyme reaction and application of method

The invention discloses a method for synthesizing phloroglucinol by virtue of in vitro enzyme reaction and an application of the method, and belongs to the technical field of biological engineering. The method comprises the following steps: respectively constructing recombinant vectors containing an acetyl coenzyme A synthetase ACS gene, an acetyl coenzyme A carboxylase subunit gene accA, an acetyl coenzyme A carboxylase subunit gene accB, an acetyl coenzyme A carboxylase subunit gene accC, an acetyl coenzyme A carboxylase subunit gene accD and a polyketone synthetase gene phlD; respectively converting the recombinant vectors into host bacteria, and then performing over-expression; crushing the bacteria by using ultrasonic waves; performing separation and purification to obtain an enzyme solution; and mixing the enzyme solution with reaction substrates in proportion and then performing in vitro enzyme reaction to synthesize phloroglucinol. By using the method disclosed by the invention, the phloroglucinol is synthesized by virtue of in vitro enzyme reaction, and the used amounts of each enzyme and accessory factors can be accurately adjusted and controlled.