Effect of extracts from Stachys sieboldii Miq. on cellular reactive oxygen species and glutathione production and genomic DNA oxidation

Objective: To evaluate the antioxidant activity of extracts and fractions from Stachys sieboldii Miq., and to examine its effect on the cellular reactive oxygen species (ROS) and glutathione (GSH) production and genomic DNA oxidation in HT-1080 cells. Methods: The ROS generation induced by H 2 O 2 was measured by the dichlorofluorescein-diacetate assay. GSH levels were measured using a fluorescent method with mBBr. Genomic DNA oxidative damage was measured with levels of oxidative DNA induced by the reaction of ferritin with H 2 O 2 . Results: The n -hexane, 85% aqueous methanol and n -butanol fractions (0.05 mg/mL concentrations) inhibited H 2 O 2 -induced ROS generation by 63%, 35% and 45%, respectively. GSH levels were significantly increased in both acetone+methylene chloride and methanol extracts ( P n -hexane significantly increased GSH levels at concentrations of 0.05 mg/mL ( P P Conclusions: These results indicate that cellular oxidation was inhibited by the n -hexane fraction and this fraction may contain valuable active compounds.