[Expression of ghrelin and its receptor GHS-R in the hypothalamus and gastrointestinal tract in rats with chronic renal failure].

Objective To investigate the expression of ghrelin and its receptor,growth hormone secretagogue receptor(GHS-R),in the hypothalamus and gastrointestinal tract in rats with chronic renal failure(CRF) and explore their relationship with the disorder of gastrointestinal tract motility.Methods SD rats were randomly divided into sham-operated group(n=8) and CRF group(n=16),and in the latter group,the rats were subjected to 5/6 nephrectomy to induce CRF.Real-time PCR and immunohistochemical staining were used to detect the distribution of mRNA and protein of ghrelin and GHS-R in the gastric fundus,duodenum,and hypothalamus.Results The rats in the CRF group showed a significantly higher expression of ghrelin mRNA and protein in the gastric fundus but a lower expression in the hypothalamus than those in the sham-operated group(P0.01),but the expression in the duodenum was similar between the two groups(P0.05).The expression of GHS-R mRNA and protein in the gastric fundus was significantly higher in the CRF group than in the sham-operated group(P0.01),while in the hypothalamus and duodenum,the expression was significantly lower in the CRF group(P0.01).Conclusion The different distribution patterns of ghrelin and GHS-R in the tissues may be an important pathological basis of gastrointestinal motility disorder in CRF.