2-Methyladenosine-Substituted 2′,5′-Oligoadenylates: Conformations, 2-5A Binding and Catalytic Activities with Human Ribonuclease L.
2010
Abstract 2-Methyladenosine-substituted analogues of 2-5A, p5′A2′p5′A2′p5′(me2A), p5′(me2A)2′p5′A2′p5′A, and p5′(me2A)2′p5′(me2A)2′p5′(me2A), were prepared via a modification of a lead ion-catalyzed ligation reaction. These 5′-monophosphates were subsequently converted into the corresponding 5′-triphosphates. Both binding and activation of human recombinant RNase L by various 2-methyladenosine-substituted 2-5A analogues were examined. Among the 2-5A analogues, p5′A2′p5′A2′p5′(me2A) showed the strongest binding affinity and was as effective as 2-5A itself as an activator of RNase L. The CD spectra of both p5′(me2A)2′p5′A2′p5′A and p5′A2′p5′A2′p5′(me2A) were superimposable on that of p5′A2′p5′A2′p5′A, indicative of an anti orientation about the base-glycoside bonds as in naturally occurring 2-5A.
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