Re-analysis of "Peptidomic analysis of cartilage and subchondral bone in OA patients".

In the May 2019 issue of European Journal of Clinical Investigation, Gatenholm et al. reported on "a method for directly analyzing osteochondral samples straight out of the operating room without cell culturing, thereby enabling identification of potential peptide biomarkers to better understand the mechanisms involved in the development of osteoarthritis (OA) and pain"(1) . Six Samples from patients were investigated, 3 from wounded (WO) and 3 from macroscopically unwounded zones (UOA) of the femur condyle, manifesting OA based on total knee arthroplasty (TKA). Using peptidomics and Tandem Mass Tag (TMT) labeling, the authors in their study reported the identification of 6296 endogenous peptides derived from 915 proteins (889 protein groups) across samples.