Growth inhibition effect of immobilized pectinase on Microcystis aeruginosa

To confirm the growth inhibition effect of immobilized pectinase on algae,co-cultivation method was used to investigate the effect of immobilized pectinase on the growth of Microcystis aeruginosa.After co-cultivation,the damage status of the algae was observed through electron microscope,and the effect of immobilized pectase on the physiological and biochemical characteristics of the algae was also measured.The results showed that the algae and immobilized pectase co-cultivated solution etiolated distinctly on the third day and there was a significantly positive correlation between the extent of etiolation and the dosage as well as the treating time of the immobilized pectinase.Under electron microscope,plasmolysis was found in the slightly damaged cells,and the cell surface of these cells was rough,uneven and irregular;the severely damaged cells were collapsed or disintegrated completely.The algal yield and the chlorophyll a content decreased significantly with the increase of the treating time.The measurement of the malondiadehyde(MDA) value showed that the antioxidation system of the treated algal cells was destroyed,and their membrane lipid was severely peroxidated.The study indicated that the immobilized pectinase could efficiently inhibit the growth of M.aeruginosa,and the inhibitory rate reached up to 96%.