Chemical modification of proteolytic enzymes by low molecular weight agents

: Low-molecular modification of proteolytic enzymes with aldehydes and anhydrides of carboxylic acids as well as with 2,4,6-trinitrobenzene sulphonic acid was studied. Specific activities of the enzymes were found to be dependent on the modification degree of their amino groups. The retaining of high activities in the region of low extents of enzyme modification enabled biocatalysts with activities similar to those of the native enzymes to be prepared.