ESI-MS/MS方法检测拟南芥膜脂分子对机械伤害的响应

This paper employed a sensitive approach based on electrospray ionization tandem mass spectrometry (ESI-MS/MS) to comprehensively analyze lipid composition changes in response to mechanical wounding in Arabidopas thaliana. The changes of 6 phospholipid classes, 2 glycolipid classes, and 3 lysophospholipid, and 120 lipid molecular species in total depicted the basic tendency of membrane lipids in response to wounding for 0, 15, 30, and 60 min in Arabidopsis thaliana. The results showed that wounding can lead to increase phosphatidic acid (PA) and 3 lysophospholipid and decrease glycolipids of the major chioroplast plastidic lipids. The increasing speed and intensity of PA produced by various lipids are very different, which shows those lipids have experienced different biochemistry processes. (1) The accumulation of 34:4 PA derived from plastidic lipid 34:4 PG (phosphatidylglycerol) is significantly slower than that of other PA species; (2) 34:6 PA accumulated a little and possibly derived from the 34:6 MGDG or DGDG. However, the content of both glycolipids has apparently decreased, which indicated they are passibly involved in other reactions. No remarkable changes of lipid mole percentage were observed.