Method for simultaneously determining three alkaloids in granules for eliminating phlegm and stopping cough for children

The invention relates to a method for simultaneously determining three alkaloids in granules for eliminating the phlegm and stopping the cough for children. The method is characterized in that the HPLC (High Performance Liquid Chromatography) method is employed for the first time, an ordinary gradient elution and reversed-phase chromatographic column are adopted, and acetonitrile, methanol and 0.1% phosphoric acid in the volume ratio of (1.5-2.5) : (12.5-11) : (86-86.5) are taken as the mobile phase; the detection wavelength is 205 nm; and the contents of ephedrine hydrochloride, cephaeline hydrochloride and emetine hydrochloride in the granules for eliminating the phlegm and stopping the cough for children are determined simultaneously, so as to end the history of no HPLC determining method for emetine and no quantitative determining indexes for granules for eliminating the phlegm and stopping the cough for children. The method provided by the invention comprises the steps of performing an ultrasonic treatment to a sample with methanol, sucking a certain amount of subsequent filtrate, removing impurities with an alumina column, and determining. The method has the benefits that crest separation of the three alkaloids is excellent, the baseline is stable, and 30 min is required to finish the determining; the quality control aim of being simple, convenient, quick, scientific, standard and multi-component quantitive by one maker is realized; and safety and effectiveness of taking granules for eliminating the phlegm and stopping the cough for children are ensured.