Mechano-growth Factor Expression in Colorectal Cancer Investigated With Fluorescent Gold Nanoparticles
2019
Background/aim: Fluorescent gold nanoparticles demonstrate strong
photoluminescence, photostability, and low cellular toxicity, making them attractive
agents for biomedical applications. Mechano-growth factor (MGF) is an isoform of
IGF1 and its expression has been demonstrated in malignancies including prostate
cancer. Materials and Methods: Near-infrared-emitting gold nanoparticles (AuNPs)
were synthesized and conjugated to MGF. Following characterization and
confirmation of conjugation, these AuNPs were used to investigate the expression of
MGF in colon cancer cell lines (HT29 and SW620) and tissues comparing normal
and colon cancer. The prostate cancer cell line PC3 and adenocarcinoma tissues
were used as positive controls. Results: Colon cancer cell lines, adenocarcinoma
tissues and polyp tissues demonstrated evidence of MGF peptide expression, which
was not found in normal colon tissues and the Human Umbilical Vein Endothelial
Cell line (HUVEC). Conclusion: MGF appears to be overexpressed in colon cancer
tissues, offering a potential unique target for imaging and drug delivery in colon
cancer.
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