The expression and significant of TSP-1 in peripheral blood monocyte of patients with IgA nephropathy

Objectives To determine the expression of thrombospondin-1 (TSP-1) in peripheral blood monocyte of patients with IgA nephropathy by the technique of flow cytometry and to explore the association between fibrosis in renal tissue and TSP-1 expression in peripheral blood monocyte of patients with IgA nephropathy.Methods The data of clinical and renal pathology,including light microscope and immunofluorescence,were studied in 30 patients with biopsy-proven IgA nephropathy.The renal pathology was analyzed according the Oxford classification of IgA nephropathy with double blind.And at the same time,TSP-1 expression in the peripheral blood monocyte were determined by the technique of flow cytometry to calculate the percentage of PST-1 positive cells.Results In the four main pathological index,the positive rates of TSP-1 in peripheral blood monocyte were associated with segmental glomerulosclerosis and segmental glomerulosclerosis,but not mesangial hypercellularity and endocapillary hypercellularity.Conclusions In the patients with IgA nephropathy,the expression of TSP-1 in peripheral blood monocyte may be a clinical index of renal chronic fibrosis. Key words: Glomerulonephritis, IGA ;  Monocytes;  Thrombospondin 1