Optimization of Grape Chromosome Specimen Making Technology

The best way to solve the resistance difference problem of existing varieties is to make use of the excellent resistance gene in Chinese Wild Vitis shape and good quality cultivation varieties to cultivate high-quality,strong-resistant new varieties.Grape chromosome identification is the foundation for the induction of polyploidy and hybrid breeding.Using the wall degradation hypotonic-tabletting method and improved phenol fuchsin staining method,grape chromosome were identified in the most suitable material and method in grape buds,leaves,stem tip,and apical tendril material.The results show that the bud and root have the most mitotic metaphase cell number,which is most suitable for chromosome flaking material,followed by the tendrils,the stem tip and the young leave tissues.Taking into consideration the difficulty in sampling and the complex degree of the reagent and program method,bud is most suitable for the wall degradation hypotonic-pressure method,and the apical most suitable for improved phenol fuchsin staining method.Thus it provides the most effective reference for grape chromosome identification.