100 Liter Transient Transfection

This is the first report of a successful 100 Liter scale transient transfection in a standard stirred bioreactor. More than half a gram of a monoclonal antibody (IgG) was produced in less than 10 days using a technology called large-scale transient gene expression (LS-TGE). Suspension adapted HEK 293 EBNA cells were transfected within a 150 1 (nominal) bioreactor by a modified calcium phosphate co-precipitation method using a total of 76.3 mg of plasmid DNA.