Cytotoxicity of two dental materials on fibroblasts derived from human embryonic stem cells

Objective: To investigate the cytotoxicity of calcium hydroxide(CH) and composite resin on fibroblasts derived from human embryo body fibroblasts-H9(EBf-H9),human dental pulp cells(hDPCs) and immortalized fibroblasts L929;and to evaluate the use of EBf-H9 as a cellular model for cytotoxicity screening of dental materials.Methods: The EBf-H9 cells were derived from human embryonic stem cells(H9) via outgrowth of embryonic body(EB);hDPCs were isolated from healthy dental pulp,and identified by immunochemical staining.Cell Counting Kit-8(CCK-8) assay was applied to analyze the cytotoxicity of CH and composite resin with serial concentrations on the 3 kinds of cells.Results: Following 24 h and 48 h(or 72 h) post-treatment of CH and composite resin,the viability of L929 cells was significantly lower than that of EBf-H9 and hDPCs(P0.05),and there was no significant difference between the last two groups(P0.05).Conclusion: Immortalized fibroblasts L929 cells exhibited different response to CH and composite resin compared with EBf-H9 and hDPCs,and the last two cell types were similar to each other.This study indicated that fibroblasts derived from human embryonic stem cells were a potential cellular model instead of traditional immortalized murine cell line for cytotoxicity screening assay.