Automated "Gamma-Flo" Radioimmunoassayof UrinaryCyclic AMP

method forcyclicAMPinurine,involving theautomatedradioimmunoassay concept reported byBrooker eta!.(7),whichisnowavailable inacommercial configuration knownasthe“Squibb Gamma-Flo.” Themethod isbasedontheoriginalmanualmethodofSteinereta!.(8),whofirstraisedantibodies withhighaffinity andspecificity towards cyclicnucleotidesanddemonstratedtheutilityofaradioimmuno-assayforcyclicnucleotides, theacetylation modification thatincreases theassaysensitivity (9),andtheinitialdescriptionoftheautomated method intheoriginaldescription ofthe“Gammaflow” concept (7).Methods forpreparing there-agents,forperforming