A discussion of anti-Aspergillus niger glucose oxidase monoclonal antibody reactivity to red blood cells of several species.

We observed that mouse spleen cells form roselles with autologous red blood cells (RBCs) and that roselle-formation was suppressed by anti-Aspergillus niger glucose oxidase monoclonal antibody (mAb)H In the present study, we investigated whether RBCs of species besides mice have the structure recognized by anti-A. niger glucose oxidase mAb by using rosette-formation and complement-mediated hemolysis. Lysmes of monkey and human RBCs did not suppress rosette-formation whereas autologous (mouse), rat and sheep RBC Iysates partially suppressed rosette-fonnation. Those Iysates exerted their suppressive activity after they had been treated at 56°C for 30 min. A. niger glucose oxidase also suppressed rosette-formation with or without treatment at 56°C for 30 min. Alternatively. anti-A. niger glucose oxidase mAb lysed mouse, rat and sheep RBCs but not human RBCs with complement. These findings suggest that the cell surfaces of mouse. rat and sheep RBCs have a structure which can be recognized by anti-A. niger glucose oxidase mAb while the cell surfaces of monkey and human RBCs do not.