Aflatoxin, proximate composition and mineral profile of stored broiler feed treated with medicinal plant leaves

Summary Objectives In the present investigation, the Morus alba ( M. alba ), Vitis vinifera ( V. vinifera ) , Ficus religiosa ( F. religiosa ) and Citrus paradisi ( C. paradisi ) leaves anti-aflatoxigenic activities were evaluated in Aspergillus flavus ( A. flavus) inoculated feed. Methods The broiler feed inoculated with A. flavus was treated with selected medicinal plant leaf powder (5%, 10% and 15% w/w) and stored for the period of six months at 28 °C and 16% moisture. The aflatoxins (AFTs) were estimated at the end of each month by Reversed Phase High Performance Liquid Chromatography (RP-HPLC) method along with proximate composition and mineral contents. Results Plant leaves controlled AFTs efficiently without affecting the feed proximate composition and mineral contents. The M. alba leaves completely inhibition (100%) the AFTs (B 1 and B 2 ) in feed at very low concentration (5%). Other plants also showed significant ( P Conclusion Based on promising efficiency of selected medicinal plant leaves, A. flavus produced AFTs could possibly be controlled in stored poultry feed.