Determination of colchiane in Iphigenia indica Kunth et Benth. by HPLC

Simulataneous HPLC determine the colchicine in different part of bulb of Iphigenia indica Kunth et Benth.and the wild population of the northwestward of Yunnan province and Yuexi county in Sichuan.The result shows:The up-ground parts of Iphigenia indica Kunth et Benth.contain few colchicine.The colchicine gradually reduce from outside to inner in the bulb of Iphigenia indica Kunth et Benth.,the outer-epidermis contain 0.134 %,the outer of bulb contain 0.171 %.The content of colchicine in the twenty-two wild populations GS01 is 0.61 % and GS22 is 0.13 %.The average conents of these colchicine is 0.40 %.