CD40-1C/T基因多态性与急性冠脉综合征易感性的研究

Objective To investigate the correlation between the CD40 gene (-1 C/T) single nucleotide polymorphism (SNP) and acute coronary syndromes (ACS), and the expression of CD40 on platelets. Method A total of 562 patients with ACS confirmed by coronary angiography were divided into 3 groups according to the clinical characteristics, namely ACS patients (n=210), stable angina (SA) patients (n=189) and control group (n=163). ACS was defined as ischemic chest pain at rest resulting in admission to hospital and ＞50% stenosis in a major coronary artery with or without a rise in troponin Ⅰ. SA was defined as stable effort-related angina without change in angina pattern in 3 months. Patients with infection, tumor, or liver or kidney disease were exclude The gene polymorphism was measured by the polymerase chain reaction and restriction fragment length polymorphism (PCB-RFLP) and identified by sequencing. The expression of CD40 on platelets was detected by how cytometry. The frequency distribution of genotypes was compared using cross-tabulation and standard χ^2 test. Result The CC genotype (31%) and C allele of frequency (57.9%) of CD40 gene in ACS patients were significantly higher than those in SA (15.9%, 43.1%) and control groups (16.1%, 42.6%). No significant difference of the genotypes or allele frequencies was found between SA and control group (χ^2=0.053, P=0.974; χ^2=0.017, P=0.897). The expression of CD40 on platelets in patients with C alleles carries was significantly higher than that of T allele carries in each group (P＜0.0001). Conclusions CD40-1 C/T polymorphism was associated with ACS in Chinese Han nationality.