Elicitation triterpene yield in Alstonia scholaris cell cultures via synergetic organisms

ABSTRACTCell cultures of Alstonia scholaris were treated with homogenates of Candida albicans, Fusarium oxysporum, Penicillium avelanium and Saccharomyces cerevisiae. The impact caused by the concentration, exposure time and the type of synergetic organisms on the accumulation of pentacyclic triterpenes was observed. When exposed to biotic elicitors for longer periods, some cell lines doubled the production of those triterpenes. S. cerevisiae homogenate was the best elicitor of triterpenes in all cell lines investigated.