Comparison of Human Cord Blood HematopoieticStem Cells Transplantation Intra-Rat Embryos York Sac Cavity and Abdominal Cavity

Objective:we used human cord blood hematopoietic stem cells in utero transplantation through yolk sac and fetal abdominal cavity of rats, and compare the complications and the engraftment rate. Methods: Human cord blood (MNC) cells were transplanted into the yolk sac and fetal abdominal cavity of rats, and the complications and the outcome of pregnancy were observed. The condition of engraftment was detected with flow cytometry assay and immunohistochemistry methods after the fetus were born. Results:The incidence of complication of group fetal abdominal cavity was higher than that of group yolk sac (P 0. 001). The fetal loss rates of group fetal abdominal cavity were higher than that of group yolk sac (P 0. 05). Human CD3 cells were detected in rats and the engraftment rate were 89. 7% and 64. 0% .respectively. There were significant difference between them (P0. 05). Human CD3,CD20 and CD34+ cells were also determined in liver,spleen and thymus of rats at 2 months of age. Conclusion: Human cord blood cells transfused into the yolk sac and fetal abdominal cavity were engrafted. Some complications occurred during operations which affected the outcome of pregnancy. Yolk sac is a better way for in utero transplantation.