Differential Regulation of Interlukin-1α and Interleukin-1β in K-562 Cells
1998
Interleukin (IL)-1α and IL-1β are encoded by two separate genes, but both function as comitogens for lymphocyte activation. In this study, we observed K-562 cells to express constitutively mRNA for IL-1α, although IL-1α was not detected in the growth-conditioned medium (GCM). However, IL-1β mRNA was not expressed unless the cells had been treated with phorbol myristate acetate (PMA). Both IL-1α and IL-1β were detected in the GCM after the cells had been cultured with PMA, suggesting that IL-1 elaboration required PMA treatment. The K-562 cells treated with PMA differentiated to the myeloblastic stage, as observed by nuclear morphologic properties by electron microscopy. PMA treatment induced de novo expression of CD61 or gpIIIa, a marker associated with megakaryoblasts. These results showed that although K-562 cells constitutively expressed IL-1α mRNA, PMA treatment was required for secretion. On the other hand, both the expression and secretion of IL-1β required treatment with PMA. This study showed that...
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