Protective effects of Sipunculus nudus polysaccharides on rats injured by low-dose irradiation combined with carbon monoxide, benzene and noise

Objective　To investigate the protective effects of Sipunculus nudus polysaccharides (SNPS) on rats injured by low-dose irradiation combined with carbon monoxide, benzene and noise. Methods　Fifty SD rats were randomly divided into normal control group, model control group, 70mg/(kg·d) SNPS group (SNPS 70 group), 140mg/(kg·d) SNPS group (SNPS 140 group) and 280mg/(kg·d) SNPS group (SNPS 280 group). SNPS was administrated intragastrically once a day before γ irradiation for 7 days. Model control group were given the same volume of 0.9% NaCl. Seven days later, all the rats were sacrificed. Peripheral blood cells were analyzed by auto blood cytometry. DNA in bone marrow cells was determined by ultraviolet spectrophotometry. The activities of superoxide dismutase (SOD) and the contents of malondialdehyde (MDA) in serum were detected by the reagent kits. The indexes of main organs (liver, spleen and thymus) were also calculated. Results　Compared with model control group, peripheral blood PLT, RBC, HCT and HGB increased significantly (P < 0.05 or P < 0.01), WBC increased a little, SOD activity and DNA in bone marrow increased significantly in SNPS groups, while the content of MDA decreased in SNPS groups compared with that in model control group (P < 0.05). No significant change was found of the main organs (liver, spleen and thymus) indexes. Conclusion　SNPS may take a protective effect on rats injured by deletion environment factors with increasing WBC and PLT in serum, improving antioxidant activity and promoting the repair of injured bone marrow.