Multi-sorting-area structure for cell sorting and use method of multi-sorting-area structure

The invention discloses a multi-sorting-area structure for cell sorting and a use method of the multi-sorting-area structure, belonging to the field of microfluidic chips. The multi-sorting-area structure consists of an external interface layer, a PDMS (polydimethylsiloxane) thin film layer and a substrate layer which are bonded together, wherein the external interface layer comprises cell sap interfaces at two ends and N groups of ventilation interfaces in the middle; the PDMS thin film is provided with N air chambers which are formed by sinking inwards; the substrate layer is provided with (N+1) groups of cell sorting areas, and each cell sorting area is provided with a sorting hole of which the diameter is di (i=1,2,...,N+1); and adjacent sorting areas are communicated by N miniature pipelines. In the process of cell sorting by the multi-sorting-area structure, as different groups of sorting areas are independent structurally, when the sorting holes in the front group of sorting area are blocked, the back sorting group is not influenced; and by combination of different number of groups of sorting areas, multiple times of sorting on cells with different sizes can be achieved, and (N+1) groups of sorted cells with sizes matching the conditions that d is less than d1, d is more than or equal to d1 and less than d2, ..., and d is more than and equal to dN and less than d(N+1) are obtained.