The scanning gradient Fourier transform (SGFT) method for assessing sarcomere organization and alignment

2020 
Recent advances in stem cell biology have allowed researchers to efficiently produce large numbers of cardiomyocytes from various pluripotent cell sources. Unfortunately, these cells exhibit properties that are characteristics of immature cardiomyocytes such as poor sarcomere organization, limited calcium handling, and reduced cell size and alignment. Specifically, the actin–myosin motor proteins that form sarcomeres within these cardiomyocytes fail to produce large, highly ordered repeating structures that are distinctive for adult myocytes. Instead, these cells produce heterogeneous sarcomeres that vary in thickness, alignment, and level of organization. Additionally, a large number of cardiomyopathies have been linked to mutations in genes encoding for sarcomeric proteins, resulting in disrupted sarcomere organization. This research focuses on a series of algorithms that provide a quantitative analysis technique to characterize the alignment and organization of sarcomere structures within aggregates an...
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