Use of angular rotors for analysis and fractionization of calf thymus DNA

Behaviour of calf thymus heterogenous DNA was studied in the angular rotor Ti50 of the preparation ultracentrifuge. A drop in DNA polymericity from 8-10(6) to 4-10(5) daltons and decrease in the amount of the applied material result in detecting the known satellite component with delta1 = 1.714 g/cm3, the value of the floating density of the main peak takes on the "true" value delta = 1.699 g/cm3. The method of digging up affects the symmetry of polymeric sample profiles. The satellite component is not found when digging up from the tube bottom. The values of GG content of the light and "heavy" fractions calculated from their floating densities during repeated centrifugation in the angular rotor and from fusing temperatures are in good agrrement.