New steroidal alkaloids from Sarcococca saligna.

Five new pregnane-type steroidal alkaloids (1-5) have been isolated from Sarcococca saligna. A combination of UV, IR, MS, and 1D and 2D NMR spectroscopic studies established their structures as salignarine A [(20S)-2β-hydroxy-4β-acetoxy-5α,6α-epoxy-20-(dimethylamino)-3β-(tigloylamino)pregnane] (1), salignarine B [(20S)-2β-hydroxy-20-(dimethylamino)-3β-(tigloylamino)-pregn-5-ene] (2), salignarine C [(20S)-2β-hydroxy-20-(dimethylamino)-3β-(senecioylamino)-pregn-5-ene] (3), salignarine D [(20S)-20-(dimethylamino)-3β-(senecioylamino)-5a-pregn-16-ene] (4), and salignarine E [(20S)-20-(dimethylamino)-3β-(tigloylamino)-pregn-4-ene] (5), respectively.