Variation of fitness and reproductive strategy in male Bufo raddei under environmental heavy metal pollution

Abstract Environmental pollution is known to adversely affect amphibian reproduction and survival, however, the knowledge of environmental heavy metal pollution on fitness of male amphibian is largely unknown. The present study aimed to explore the variation in fitness of male Bufo raddei , a widespread anuran in northwest China, subjected to long-term heavy metal stress in Baiyin (BY) city. BY is heavily polluted by heavy metals mainly copper, zinc, lead and cadmium; meanwhile, Liujiaxia (LJX), a relatively unpolluted area, was chosen as control. Differences in advertisement call, larynx size, breeding glands size, as well as forearm force during courtship and amplexus in male B. raddei between the two populations were analyzed. The results revealed a competitive advantage in advertisement call in BY population, together with larger breeding glands size and forearm force, which demonstrated a relatively higher fitness. Using skeletochronological analysis, we found that more than 40% of males from BY began to breed at 2 years old, which was only 6.93% for males from LJX. Correspondingly, the average age for all males participate in breeding was younger from BY than from LJX. Not surprisingly, males from BY showed a relatively lower body condition. All these results illustrated males from BY invested more in reproduction to increase fitness at the cost of health and survival. This reproductive trade-off might ultimately influence stability of B. raddei population because of the environmental heavy metal pollution.