Natural compounds from Leeamacrophyllaenhance phagocytosis and promote osteoblastsdifferentiationbyAlp, Type 1 collagen and Osteocalcin gene expression.

The current study investigated the immunomodulating and osteoblast differentiation potential of the natural compounds from Leeamacrophylla(LMN). Immunomodulatory effects have been investigated by the phagocytosis of Candida albicans using polymorphonuclear neutrophil cells in thein vitro slide method. A bioactivity-guided fractionation technique was used toevaluate thestimulating effect of L. macrophyllamethanol extract on osteoblast differentiation using mouse osteoblastic cells.A low dose of LMN was found to stimulate the phagocytic effect better than a higher dose. The natural compounds from L. macrophyllahave significantly increased alkaline phosphatase (ALP) and osteocalcin activities. The LMN promoted the osteoblast differentiation through up-regulation of ALP, osteocalcin,and type 1 collagen in a dose-dependent manner. These natural compounds also upregulated Alp, osteocalcin,and Type 1 collagengene expressions. The data suggest that LMNhas potential anabolic sequel on bone formation and osteoblast differentiation.