1480 LYMPHOCYTE SUBPOPULATIONS IN LIPOID NEPHROSIS (LN)

Previous studies in LN have suggested an immunologic pathogenesis. We have characterized B and T cell subpopulations from peripheral blood lymphocytes(PBL) of 10 patients with LN. B lymphocytes displaying different isotypes were detected by staining with fluorescein(Fl) and Rhodamine(Rh) conjugated, affinity-purified goat anti-human μ, σ, γ, or α. T lymphocytes were evaluated using monoclonal mouse anti-human T cell reagents(OKT3 = total T, OKT4 = inducer T, OKT8 = suppressor/cytotoxic T). Plasma cells(PC) were stained after fixation with Fl or Rh goat anti-human μ, δ, γ, or α.