Neuroprotective effects of a Rhodiola crenulata extract on amyloid-β peptides (Aβ1-42) -induced cognitive deficits in rat models of Alzheimer's disease

Abstract Background Rhodiola crenulata has been wildly used as a healthy food, antidepressant and antifatigue for many years in China. Recent studies suggested that Rhodiola crenulata extract (RCE) has cognitive protective effects in the treatment of Alzheimer's disease (AD). Purpose To assess the protective effects of RCE on cognitive deficits and clarify its therapeutic mechanisms in Aβ 1-42 -induced rat models of AD. Study design RCE was prepared by freeze-drying technology. Their protective effects on Aβ 1-42 -induced rat models of AD and the preliminary therapeutic mechanisms were studied. Methods The Y maze test and Morris water maze (MWM) test were conducted to evaluate the learning and memory abilities of the rats. Subsequently, biochemical assays, hematoxylin-eosin staining, immunohistochemistry and Western blotting were performed to elucidate the mechanisms. Results RCE significantly increased the spontaneous alternation (F (6, 111) = 8.165, p p p p 1-42 injected rats. Moreover, RCE increased the superoxide dismutase (SOD) activity and decreased the malondialdehyde (MDA) level in cortex of Aβ 1-42 injected rats (F (6, 34) = 5.097, p p p p Conclusion Our findings demonstrated that RCE significantly alleviated the learning and memory deficits in the Aβ 1-42 -induced rat models of AD. The mechanisms involved its protection effects against cholinergic system deficiency, oxidative stress damage and GSK3β activation. RCE may be a potential therapeutic medicine with multi-targets to prevent the progression of cognitive deterioration in AD.