A simple method for preparing meiotic chromosomes from mammalian testis

Freshly removed testis tubules are treated with hypotonic citrate solution and fixed in 3∶1 acetic alcohol without undue rupture. The material is transferred to 60% acetic acid and the resulting suspension is air-dried onto warm slides using a micropipette. Finally the slides are stained with lacto-acetic orcein. — Provided suitable storage is made, slides need not be prepared in the same week as fixation and this may facilitate the collection of material during field study.