Large Scale HPLC Purification of Oligodeoxyribonucleotides for Studies of the Binding of CC-1065 to DNA by NMR Spectroscopy

Abstract We have investigated four different HPLC columns and several conditions in search of the most efficient procedure for purification of milligram amounts of DNA. We are reporting the optimal conditions for a preparative purification using a Dupont Zorbax Bioseries Oligo column.