대하(帶下)처방 3종의 항염, 항소양, 항균 효능에 관한 실험 연구 : 은화사간탕(銀花瀉肝湯), 소복축어탕(少腹逐瘀湯), 완대탕(完帶湯)

Objectives: The purpose of this study was to investigate the in vitro anti-inflammatory, anti-pruritic and antimicrobial effects of the three herbal prescription (EST, SCT, WDT), which has been traditionally used for treating leukorrhea induced by various infections in the female genital tract. Methods: In this experiment, the anti-inflammatory effects were evaluated by Nitric oxide (NO), Interlukine-lβ (IL-lβ), Interlukine-2 (IL-2), Interlukine-6 (IL-6), Tumor necrosis factor-a (TNF-a), Prostaglandin E2 (PGE2), Leukotriene B4 (LTB4 production amount and Inducible nitric oxide synthase (iNOS), Nuclear factor kappa B (NF-κB), Cyclooxygenase-2 (COX-2) gene expression levels in RAW264.7 cells. And the anti-pruritic effects were evaluated by Histamine, Acetylcholine (ACh), Acetylcholinesterase (AChE), Substance P production amount in Mast cell/9 (MC/9) and Pheochromocytoma 12 (PC12) cells. The anti-microbial effect was measured by inhibition zone diameter on coli, Staphylococcus minis, Pseudomonas aeruginosa and niger. Results: As a result of measuring anti-inflammatory efficacy, IL- lβ, IL-2, IL-6, TNF-a, PGE2, and LTB4 production amounts were significantly reduced in the EST, SCT, WDT extraction groups compared with the control group, and significantly decreased the amount of NF-kB, iNOS, and COX-2 gene expression and the amount of Phospho-Inhibitor kappa B alpha (p-IκB-a)/Inhibitor kappa B alpha (IκB-a) and NF-B p65 protein expression. In addition, As a result of measuring the anti-pruritic effect, the amounts of histamine, ACh and Substance P were significantly decreased, and AChE production was slightly decreased, but it’s significance did not appear. Finally the anti-microbial effects of EST, SCT, WDT extraction groups against aeruginosa, Candida albicans and Aspergillus was inhibited, however the growth of Escherichia coli and Staphylococcus aureus was not inhibited. Conclusions: These data suggest that EST, SCT, WDT can be used to treat patients with leukorrhea.