Purification of Enolase from Human Brain Tissue

Neuron-specific enolase (NSE) and non-neuronal enolase (NNE) were purified using a small number of methods performed in the following order: DEAE-cellulose chromatography, diafiltration, preparative isoelectric focusing and gel-filtration. Pure isoenzymes were isolated in quantities, which would allow us to produce antisera against each form of the enzyme.