The effect of DDPh on the PDGH mRHA gene expression of pulmonary artery SMC induced by the HECCM

The technology of nucleic acid in situ hybridization was used to study the effect of DDPH on PDGF mRNA gene expression of pulmonary artery SMC induced by the hypoxic bovine endothelium cell condition medium (HECCM). Experimental groups included normoxic, hypoxic serum free medium group (N,H group), normoxic, hypoxic endothelium cell conditional medium groups (NECCM,HECCM groups) and NECCM+DDPH, HECCM+DDPH group. Automatic image analysis showed that HECCM substantially enhanced the expression of PDGF-A and -B chain mRNA (1.8 fold and 1.7 fold compared with N group; 1.40 fold and 1.49 fold compared with NECCM group, respectively, P<0.01). HECCM+DDPH lowered the expression of PDGF-Aand-B chain mRNA (0.73 fold and 0.65 fold compared with HECCM, with significant difference; but no difference was found when compared with NECCM). The results suggested that PDGF may be an autocrine growth factor of PASMC induced by HECCM and play an important role in the vascular structural remodeling of hypoxic pulmonary hypertension. DDPH could inhibit multiplication of PASMC induced by HECCM at the level of PDGF gene transcription.