Separation and Purification of Extracellular Polysaccharide from Biofouling Layer of MBR

Separation and purification of extracellular polysaccharide(EPS) from biofouling layer of MBR were investigated.The results showed that the amount of EPS extracted by thermal treatment(80 ℃,30 min) was 86%.The protein was removed by Sevage method.The EPS was further purified by cellulose chromatography.The purified sample EPS-A1 was separated by Sephacryl S-400 HR column chromatography.Analysis of polysaccharide by UV spectrum showed that there were no absorption peaks of protein and nucleic acid at 280 nm and 260 nm.FT-IR results showed the EPS-A1 had typical absorption peaks of polysaccharide.