Cloning and sequence analysis of isopentenyltransferases gene from Cucumis sativus.

Primers were designed according to the ATIPTs genes sequence of Arabidopsls thaliana in GenBank.Partial sequences of the conserved region of isopentenyltransferases gene were cloned from cucumber S94 line by PCR methods,and then full-length sequences were obtained by filter the genomic BAC library from cucumber.Sequencing analysis showed that the gene(GenBank accession number HQ326777) was 1 005 bp length,encoded 334 amino acids,and included ATP/GTP binding site(GATGTGKS).Homology analysis showed that the nucleotide and deduced amino acids were highly homologous to ipt gene from other species,specially the highest homology to ipt gene of Populus trichocarpa with the homology to 65%.According to the results of sequence analysis of cucumber ipt gene obtained in the study,we confirm that we have cloned ipt gene from Cucumis sativus,named cs-ipt.