Synthesis and activity of phthalylglutamic acid analogs as potential folic acid antagonists in bacteria

Four phthalylglutamic acid analogs were found to have no activity as inhibitors of bacterial growth in chemically defined media. More specifically, the compounds were not active as folic acid antagonists in bacteria. Cell permeability studies on these substances have not been conducted yet; consequently, this phenomenon is not ruled out as the mechanism whereby these compounds are inactive.