The influence mechanism of DO on the microbial community and carbon source metabolism in two solid carbon source systems

Abstract The regulation mechanism of parameters on microorganisms and carbon source metabolism of solid carbon source simultaneous nitrification and denitrification (SND) process is not clear. In this paper, the effects of dissolved oxygen (DO) and biodegradable polymer (BDPs) types ((Polycaprolactone, PCL) and (Polybutylene succinate, PBS)) on treatment performance and microbial characteristics were investigated. The results show that the total nitrogen (TN) removal efficiency of SND process using PBS and PCL as fillers reached 93.02% and 97.28% under optimal parameter of DO 5 mg/L, respectively. The dominant genus with nitrogen removal performance in the PCL carbon source system are Hydrogenophaga and Acidovorax, and the main genus in the PBS system are Acidovorax and unclassified_Comamonadaceae. The co-metabolic network in PCL is more complex and easier to be regulated by DO. The BDPs types mainly affect the co-metabolic network with nodes of Thiothrix and Chryseomicrobium, ultimately leading to changes in the community structure. By comparison, BDPs types have a more significant impact on community structure than DO under low DO conditions (1 and 2 mg/L), but not under high DO condition(5 mg/L). Further, the distribution of functional enzymes may conflict between nitrification and carbon source degradation under high DO condition. Controlling the DO within the range of 2 mg–5 mg can further improve carbon source utilization efficiency.