Solid-phase extraction of midazolam and two of its metabolites from plasma for high-performance liquid chromatographic analysis.
1991
Abstract A rapid, sensitive and selective assay of midazolam and two of its metabolites in plasma, based on high-performance liquid chromatography, has been developed. The compounds are subjected to solid-phase extraction, using C 18 cartridges (Bond-Elut). Recoveries are in excess of 90% for midazolam and its metabolites. The limit of quantitation of the assay is 50 ng/ml of plasma for each compound.
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