Multiple Shoots Induction from Indigenous NigerianPumpkin (Cucurbita pepo L.)

Aim: The aim of this study is to develop a protocol for in vitro regeneration of a Nigerian indigenous pumpkin (C. pepo L.) via seedling-derived cotelydon, cotyledonary node and hypocotyl explants. Study Design: A combination of 0.00, 1.00, 2.00 and 3.00 mgl-1 of 6-Benzylaminopurine (BAP) and 0.00 or 0.05 mgl-1 of 2,4-Dichlorophenoxy acetic acid (2,4-D) for each explant type were set up in three replicates making a total of 36 culture vessels in the entire experimental set up. Five explants were cultured per combination. Place and Duration of Study: The work was conducted in the Plant Tissue Culture Laboratory of the Department of Biological Sciences, College of Science and Technology, Covenant University, Ota, Ogun State, Nigeria, between January 2017 and July 2017. Methodology: Hypocotyl, cotyledonary node and cotyledon explants derived from 4-week old