Analysis of pathogenic detection data of epidemic cerebrospinal meningitis W135 cases in Hefei,from 2012 to 2013

Objective To understand the etiological characters of the epidemic cerebrospinal meningitis W135 cases and their close contacts in Hefei from 2012 to 2013. Methods Biochemical testing,serotyping,PCR and antimicrobial susceptibility testing were performed on samples of 2 death cases and 9 close contacts. Multilocus sequence typing( MLST) for DNA sequencing was used for housekeeping gene. Results All of the 11 samples were confirmed Neisseria meningitidis serogroup W135. DNA sequencing showed that the 11 positive strains were ST- 11,which belonged to ST- 11 / ET- 37 clone complex. 5 strains had drug resistance to nalidixic acid,Ciprofloxacin and Sulfamethoxazole. Conclusion Neisseria meningitidis serogroup W135 were isolated from the death cases and close contacts of epidemic cerebrospinal meningitis,which showed flora vicissitude.