RFID Technology Serving Honey Bee Research: A Comprehensive Description of a 32-Antenna System to Study Honey Bee and Queen Behavior

The fields of electronics and information technology have witnessed rapid development during the last decades, providing significant technical support to the field of biological sciences. Radio-Frequency Identification (RFID) technology has been used to automate the monitoring of animal location and behaviors in a wide range of vertebrate and invertebrate species, including social insects such as ants and honey bees (Apis mellifera L.). This technology relies on electromagnetic fields to identify and track transponders attached to objects automatically. Implementing new technologies to serve research purposes could be time consuming and require technical expertise from entomologists and researchers. Herein, we present a detailed description on how to harness RFID technology to serve honey bee research effectively. We describe how to build and operate a 32-antenna RFID system used to monitor various honey bee behaviors such as foraging, robbing, and queen and drone mating, which can be used in other social insects as well. Preliminary data related to queen nuptial flights were obtained using this unit and presented in this study. Virgin queens labeled with ≈5 mg transponders performed multiple (one to four) nuptial/orientation flights a day (9 a.m. to 5 p.m.) ranging from 8 to 145 s each. Contrary to virgin queens, no hive exit was recorded for mated queens. At full capacity, this unit can monitor up to 32 honey bee colonies concurrently and is self-sustained by a solar panel to work in remote areas. All materials, hardware, and software needed to build and operate this unit are detailed in this study, offering researchers and beekeepers a practical solution and a comprehensive source of information enabling the implementation of RFID technology in their research perspective.