Development of a sensitive and selective LC/MS/MS method for the simultaneous determination of intracellular 1-beta-d-arabinofuranosylcytosine triphosphate (araCTP), cytidine triphosphate (CTP) and deoxycytidine triphosphate (dCTP) in a human follicular lymphoma cell line
2009
Abstract A method was developed for the quantification of araCTP, CTP and dCTP in a human follicular lymphoma cell line. This method involves solid phase extraction (SPE) using a weak anion-exchanger (WAX) cartridge, a porous graphitic carbon high-performance liquid chromatography (HPLC) column separation, and tandem mass spectrometry (MS/MS) detection. By using a triple quadrupole mass spectrometer operating in negative ion multiple reaction monitoring (MRM) mode, the method was able to achieve a lower limit of quantification (LLOQ) of 0.1 μg mL −1 for araCTP and of 0.01 μg mL −1 for both CTP and dCTP. The method was validated and used to determine the amount of araCTP, CTP and dCTP formed after incubation of araC and an araCMP prodrug in the human follicular lymphoma cell line RL.
Keywords:
- Triple quadrupole mass spectrometer
- Selected reaction monitoring
- Analytical chemistry
- High-performance liquid chromatography
- Tandem mass spectrometry
- Deoxycytidine triphosphate
- Liquid chromatography–mass spectrometry
- Arabinofuranosylcytosine triphosphate
- Chemistry
- Cytidine triphosphate
- Chromatography
- Solid phase extraction
- Correction
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