Endurance training reduces exercise-induced acidosis and improves muscle function in a mouse model of sickle cell disease

Abstract Sickle cell disease (SCD) mice (Townes model of SCD) presented exacerbated exercise-induced acidosis and fatigability as compared to control animals. We hypothesize that endurance training could represent a valuable approach to reverse these muscle defects. Endurance-trained HbAA (HbAA-END, n  = 10), HbAS (HbAS-END, n  = 11) and HbSS (HbSS-END, n  = 8) mice were compared to their sedentary counterparts (10 HbAA-SED, 10 HbAS-SED and 9 HbSS-SED mice) during two rest – exercise – recovery protocols during which muscle energetics and function were measured. In vitro analyses of some proteins involved in muscle energetics, pH regulation and oxidative stress were also performed. Exercise-induced acidosis was lower in HbSS-END mice as compared to their sedentary counterparts during both moderate ( p p In vitro analyses revealed that enolase/citrate synthase ratio was reduced in HbSS-END ( p p p