Lethal effect of high temperatures on the eggs of Fasciola hepatica.

39.9%, respectively, and these values in the group incubated at 45°C were 46%, 42.5% and 33.7%, as well. However, in the case of incubation at 50°C for 1, 2, 3, 4 and 5 h, these values were recorded as 1.96%, 0.57%, 0.07%, 0.00% and 0.00%, respectively. The results indicated that the eggs were susceptible to high temperatures and incubation of the eggs at 50°C for 4 h was enough to significantly inactivate the eggs and prevent miracidial formation (P<0.001). However, using the livestock faeces as fertilizer in many rural areas may lead to the contamination of pasture. According to the findings of this study, in the areas with a high prevalence rate of Fasciola hepatica, manure storage for a sufficient time or heating the livestock manure before use as fertilizer is strongly recommended.