Construction of a lentiviral vector for RNA interference of human VIM gene and its silencing effect in pancreatic cancer cells

Jianxin Jiang,Ming Shen,Renyi Qin,Rui Tian,Jing Li,Min Wang

Construction of a lentiviral vector for RNA interference of human VIM gene and its silencing effect in pancreatic cancer cells

2009

Jianxin Jiang
Ming Shen
Renyi Qin
Rui Tian
Jing Li
Min Wang

Objective To construct a lentiviral expression vector for RNA interference (RNAi) of human VIM gene; and assess its gene silencing effect in pancreatic cancer cell line Panc-1.

Keywords:

Vimentin
Gene silencing
Expression vector
Lentivirus
Viral vector
Gene
RNA silencing
RNA interference
Molecular biology
Biology
Pancreatic cancer
Pathology
Cancer research

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