Optimization of exopolysaccharides production by Bacillus subtilis

The environmental and nutritional parameters were studied aiming to give a maximal exopolysaccharides (EPS) production by Bacillus subtilis. The basal medium (BM) was appeared to be the optimum medium among the seven tested media at 150 rpm. The highest production of EPS was obtained after 3 days incubation period, pH 7.0 and 25C. Sucrose was the best carbon source stimulating the maximum production of EPS followed by mannitol. The maximum production was achieved at concentration 4.5% of sucrose. The highest production of EPS was recorded by using yeast extract with a concentration of 0.22%.