Study on detection of formaldehyde presenting raw milk by HPLC and FeCl3-HCl reagent.

In this paper,two methods,HPLC and FeCl3-HCl,were used to detect the presence of formaldehyde in raw milk,quantitatively and qualitatively.In HPLC method,formaldehyde was extracted by boiled water and derived by 2,4-dinitrophenylhydrazine.Agilent SB.C18 column and acetonitrile-water(65+35) were used.The wavelength was 365nm.The recovery of 87% were obtained.In FeCl3-HCl method,formaldehyde positive sample showed purple with minimum formaldehyde content at 5mg/kg.It is concluded that FeCl3-HCl method is a sensitive,simple and fast method and can be easily used to inspect the presence of formaldehyde in raw milk.